FD-FLIM
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Location: | LCAM, Room A2.34 |
| Contact person: |  |
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| 2nd Contact person: |  |
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| Techniques: | (Semi-)Widefield imaging Confocal imaging (using spinning disk) Spectral imaging microscopy (SPIM) Fluorescence Lifetime Imaging Microscopy (FLIM) Total Internal Reflection Microscopy (TIRF) |
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| Booking: | The FLIM microscope can be booked via the webcalender and is only available for trained users. For first time-users contact Dorus Gadella (+6259). | |
| Lasers: | 405 nm (diode laser) 442 nm (diode laser) 488 nm 514 nm 532 nm 568 nm For more info about laser safety, contact Ronald Breedijk (+7860) |
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| Description: The Zeiss Axiovert widefield microscope is equiped with insturments for frequency-domain FLIM or spectral imaging. The setup can be extended with a spinning disk device for confocal measurements. | Mercury lamp filters: | 365 nm 405 nm 436 nm 470 nm 500 nm 546 nm 577 nm |
| Objectives: | 10X (Plan NeoFluar NA 0.3 air) 20X (Plan NeoFluar NA 0.5 air) 40X (Plan NeoFluar NA 1.3 oil) 63X (Plan Apochromat NA 1.4 oil) 63X (AchroPlan NA 0.95 water, no coverslip, long WD) 100X (AlphaPLAN NA 1.45 oil) |
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| Microscope: | Zeiss Axiovert 200M | |
| Detection filters: | download filter-scheme (not available yet) Beamsplitters: CFP GFP YFP RFP 80/20% mirror Calibration CubeEmission filters: CFP bandpass GFP bandpass YFP bandpass RFP bandpass 400 nm longpass 430 nm longpass 460 nm longpass 500 nm longpass 525 nm longpass 570 nm longpass 600 nm longpass |
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| Accessoires: | Perkin Elmer UltraVIEW Spinning disk unit Image intensifier (Lambert Instruments) Cooled CCD camera (Coolsnap HQ) Spectrograph (400-700nm) Imspector Cooled CCD camera (Hamamatsu ORCA II) |
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| Software: | LCAM Image J macro to create colored-FLIM images | |
| Manuals: | LCAM FLIM Start-up manual (not avialable yet) LCAM FLIM Laser manual LCAM user rules LCAM startup procedure 405 laser LCAM multifrequency-FLIM procedure LCAM EB calibrationprocedure/image processing |