LCAM-FNWI
welcomes you
The Van Leeuwenhoek Centre for Advanced Microscopy (LCAM) is a collaboration between three innovative microscopy centres at the Academic Medical Centre (AMC), the Faculty of Science of the University of Amsterdam (FNWI) and the Netherlands Cancer Institute (NKI).
LCAM offers visualisation and microscopical analysis of cell organelles, cells and tissues.
The first upcoming LCAM events are:
- 10 April 2019LCAM Training day
- 9 June 2019FEBS Advanced course "Functional imaging of nuclear organisation and signaling
- 19 June 2019LCAM Training day
For more info about past and future LCAM-courses & events visit www.microscopycourse.nl
Hyperacidification by a vacuolar proton-pumping P-ATPase complex
petal cells are acidified by a different vacuolar proton transporter instead of the V-ATPase. The new pump is the heterodimer of a P3A-ATPase (PH5, very similar the plant cells plasma membrane H+-pump) and a P3B-ATPAses (PH1, similar to a bacterial Mg2+ transporter). The combination of these two proteins makes up a super-proton-transporter, which is able to acidify petal vacuoles to low pH.
The PH5 proton pump is a more powerful transporter than the V-ATPase, using more than one ATP molecule for each translocated proton. Its combination with PH1 makes it able to keep on pumping when the proton gradient (and therefore also the electrochemical gradient) becomes steep, allowing the hyper-acidification of the lumen. Read more.
The research, carried out at Plant Development and Epi-Genetics (SILS-FNWI) mainly by Pamela Strazzer & Francesca Quattrocchio has been published in the latest edition of
Nature Communications.
Would you like to display your experimental data here? Contact us!
LCAM-FNWI operates directly under the research group Molecular Cytology at the University of Amsterdam. Here you will find a wide range of instrumentation for fluorescence-, confocal-, super-resolution & living cell microscopy combined with equipment for functional imaging.
LCAM has become a Euro-BioImaging Flaghship centre for Functional Imaging and High Content Microscopy.
For more info about LCAM-FNWI:
Director: Dorus Gadella
Website: Mark Hink
For microscope-specific questions communicate with the contact person indicated in the Equipment-section