FLIMscreen Location: LCAM, Room A2.34
Contact person: staff-Dorus
2nd Contact person:
Techniques: Widefield imaging
Multi-channel ratio-imaging

Fluorescence Lifetime Microscopy (FLIM)
Multi-parameter screening
Booking: The FLIM screener microscope can be booked via the webcalender and is only available for trained users. For first time-users contact Mark Hink (+6211).
LEDs: 446 nm
470 nm
507 nm
532 nm
Objectives: 1x Plan UW, NA 0.05 (air)
10x Plan Apo, NA 0.45 (air)
20x Plan Apo, NA 0.75 (air)
40x Plan Apo, NA 0.95 (air)
60x Plan Apo, NA 1.4 (oil)
Microscope: Nikon TE
Detection filters: Download filter scheme here
Accessoires: 37oC incubation chamber
Low magnification setup for screening agar plates
Four channel optosplit unit with Orca CCD camera
Software: NIS Elements
Custom-made Matlab screening scripts
Nd2 images can be viewed with NIS Elements (Licensed), freely available NIS-Elements viewer or free ImageJ  software in combination with the Bioformats LOCI plugin or use the Fiji variant.
Manuals: LCAM FLIM screen startup manual
LCAM Data acquisition manual
LCAM user rules