FLIMscope
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Location: | LCAM, Room A2.34 |
| Contact person: |  |
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| 2nd Contact person: |  |
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| Techniques: | Widefield imaging Multi-channel ratio-imaging Fluorescence Lifetime Microscopy (FLIM) Multi-parameter screening |
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| Booking: | The FLIM screener microscope can be booked via the webcalender and is only available for trained users. For first time-users contact Mark Hink (+6211). | |
| LEDs: | 446 nm 470 nm 507 nm 532 nm |
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| Objectives: | 1x Plan UW, NA 0.05 (air) 10x Plan Apo, NA 0.45 (air) 20x Plan Apo, NA 0.75 (air) 40x Plan Apo, NA 0.95 (air) 60x Plan Apo, NA 1.4 (oil) |
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| Microscope: | Nikon TE |
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| Detection filters: | Download filter scheme here | |
| Accessoires: | 37oC incubation chamber Low magnification setup for screening agar plates Four channel optosplit unit with Orca CCD camera |
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| Software: | NIS Elements LIFA Custom-made Matlab screening scripts Nd2 images can be viewed with NIS Elements (Licensed), freely available NIS-Elements viewer or free ImageJ software in combination with the Bioformats LOCI plugin or use the Fiji variant. |
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| Manuals: | LCAM FLIM screen startup manual LCAM Data acquisition manual LCAM user rules |