Leica SP8
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Location: | Sciencepark room A2.40 |
| Contact person: |  |
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| 2nd Contact person: |  |
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| Techniques: | Confocal imaging of max. 5 channels with standard PMTs (3x) or HyDs (2x) (Semi-)Widefield imaging Standard confocal techniques like co-localization & FRAP Deep imaging FRET N&B/RICS |
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| Booking: | The microscope can be booked by trained users only. The schedule can be viewed in the webcalender. First time-users should contact Mark Hink. | |
| Lasers: | 405 nm diode laser 440 nm dpss laser 458, 476, 488 & 514 nm Argon ion laser 561 nm dpss laser 594 nm HeNe laser 633 nm HeNe laser For more info about laser safety, contact Ronald Breedijk (+7860) |
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| Objectives: | HC Plan Apo 10x, NA0.4 (air) HC Plan Apo 20x, NA0.75 (air) HC Plan Apo 40x, NA1.3 (oil) HC Plan Apo 63x, NA1.4 (oil) HC Plan Apo 63x, NA1.2 (water) Fluotar VISIR 25x, 25x NA0.95 (water) long-working distance |
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| Filters: | Download laser and filter scheme here | |
| Accessoires: | Equiped with Okolab 37oC incubation box with option for CO2 Piezo element for precise z-stacking Adaptive focusing Possibility for automatic water re-immersion of objective |
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| Software: | Leica Las-X, images can be imported into ImageJ using the LOCI plugin (automatically recognized in Fiji) | |
| Manuals: | LCAM Leica SP8 Start-up manual LCAM user rules LCAM Manual connection to data-server Las-X helpfiles available via desktop link or via Mark |