Leica SP8

Location: Sciencepark room A2.40
Contact person:
2nd Contact person:
Techniques: Confocal imaging of max. 5 channels with standard PMTs (3x) or HyDs (2x)
(Semi-)Widefield imaging
Standard confocal techniques like co-localization & FRAP
Deep imaging
Booking: The microscope can be booked by trained users only. The schedule can be viewed in the webcalender. First time-users should contact Mark Hink.
Lasers: 405 nm diode laser
440 nm dpss laser
458, 476, 488 & 514 nm Argon ion laser
561 nm dpss laser
594 nm HeNe laser
633 nm HeNe laser
For more info about laser safety, contact Ronald Breedijk (+7860)
Objectives: HC Plan Apo 10x, NA0.4 (air)
HC Plan Apo 20x, NA0.75 (air)
HC Plan Apo 40x, NA1.3 (oil)
HC Plan Apo 63x, NA1.4 (oil)
HC Plan Apo 63x, NA1.2 (water)
Fluotar VISIR 25x, 25x NA0.95 (water) long-working distance
Filters: Download laser and filter scheme here
Accessoires: Equiped with Okolab 37oC incubation box with option for CO2
Piezo element for precise z-stacking
Adaptive focusing
Possibility for  automatic water re-immersion of objective
Software: Leica Las-X, images can be imported into ImageJ using the LOCI plugin (automatically recognized in Fiji)
Manuals: LCAM Leica SP8 Start-up manual
LCAM user rules
LCAM Manual connection to data-server
Las-X helpfiles available via desktop link or via Mark