Nikon A1

microscope-A1 Location: LCAM, room A2.34
Contact person: staff-Ronald
Techniques: (Semi-)Widefield imaging
Confocal imaging
Standard confocal techniques like co-localization & FRAP
Controled Light Exposure Microscopy (CLEM)
Spectral analysis (f.e. to study FRET)
Booking: The A1 microscope can be booked by trained users only. To book the microscope, Ronald Breedijk (+7860) should be contacted. The composed measurement schedule will be published in the webcalender on Thursday, the week beforeFor first time-users contact Ronald Breedijk (+7860).
Lasers: 405 nm diode laser 
440 nm diode laser 
458 nm, Ar mW
488 nm, Ar mW

514 nm, Ar mW 
561 nm, dpss  mW
594 nm HeNe laser  
640 nm, diode laser  mW
For more info about laser safety, contact Ronald Breedijk (+7860)
Objectives: 20x ELWD
20x air/water/oil
40x oil
60x oil
Microscope: Nikon Ti + mercury and transmission lamp
DAPI, GFP, FITC and TexasRed fluorescence cubes
motorized xyz stage
Description: download filter-scheme here
Accessoires: OKO-lab incubation chamber for temperature and CO2 control. The microscope is equiped with a resonant scanner so that  images can be acquired at very high speeds (suitable for fast FRAP)
Software: Standard A1 files can be viewed with the Nikon NIS Elements (dongle required) and the free Nd2 viewer. Additional plugins can be downloaded here to import the files into ImageJ. Download an imageJ plugin (loci-tools.jar) to load Nikon tif images.
Manuals:  LCAM A1 Start-up manual
LCAM A1 Laser control manual
LCAM user rules

 

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