Zeiss Cell Observer
Location: | LCAM, Room A2.40 | |
Contact person: | ||
2nd Contact person: | ||
Techniques: | (Semi-)Widefield imaging | |
Booking: | The Cellobserver microscope can be booked via the webcalender and is only available for trained users. For first time-users contact Ronald Breedijk (+7860). | |
Description: This Zeiss widefield microscope is equiped with a lamp and 37oC incubation chamber, making it suited for multi-color experiments possibly combined with long term measurements | Excitation: | Cairn Xenon Arc lamp in combination with a computer controlled monochromator (bandwidth: 0.30 nm). |
Objectives: | 10x CP-Apochromat, N.A. 0.25 Ph1 40x Zeiss Plan-Neofluor, N.A. 1.3 Oil Ph3 100x Zeiss Plan-Apochromat, N.A. 1.4 Oil Ph3 |
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Microscope: | Zeiss Axiovert 200M + mercury and transmission lamp multiple fluorescence cubes (see detection filters) | |
Detection filters: | Download filter scheme here | |
Camera filters: | 1. empty 2. CFP BP 470-30 3. YFP BP 535-30 4. RFP BP 620-60 5. GFP BP 525-40 6. BFP BP 445/40 7. empty 8. OFP 495/30 & 568/25 9. blocked 10. blocked |
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Accessoires: | Zeiss incubation chamber for temperature and CO2 control | |
Software: | Metamorph 6.1 | |
Manuals: | LCAM Cellobserver Start-up manual LCAM user rules |