Zeiss Cell Observer

 cellobserver Location: LCAM, Room A2.40
Contact person: staff-Anna
2nd Contact person: staff-Joachim
Techniques: (Semi-)Widefield imaging
Booking: The Cellobserver microscope can be booked via the webcalender and is only available for trained users. For first time-users contact Ronald Breedijk (+7860).
Description: This Zeiss widefield microscope is equiped with a lamp and 37oC incubation chamber, making it suited for multi-color experiments possibly combined with long term measurements   Excitation: Cairn Xenon Arc lamp in combination with a computer controlled monochromator (bandwidth: 0.30 nm).    
Objectives: 10x CP-Apochromat, N.A. 0.25 Ph1
Zeiss Plan-Neofluor, N.A. 1.3 Oil Ph3
100x Zeiss Plan-Apochromat, N.A. 1.4 Oil Ph3 
Microscope: Zeiss Axiovert 200M + mercury and transmission lamp multiple fluorescence cubes (see detection filters)
Detection filters: Download filter scheme here
Camera filters: 1. empty
2. CFP BP 470-30
3. YFP BP 535-30
4. RFP BP 620-60
5. GFP BP 525-40
6. BFP BP 445/40
7. empty
8. OFP 495/30 & 568/25
9. blocked
10. blocked
Accessoires: Zeiss incubation chamber for temperature and CO2 control
Software: Metamorph 6.1
Manuals: LCAM Cellobserver Start-up manual
LCAM user rules