Zeiss LSM510

microscope-LSM510   Location: LCAM, Room A2.40
Contact person: staff-Ronald
2nd Contact person: staff-Mark
Techniques: (Semi-)Widefield imaging
Confocal imaging
Standard confocal techniques like co-localization  & FRAP
Multi-Photon Excitation (MPE)
UV excitation
Booking: The LSM510 microscope can be booked by trained users only. To book the microscope Ronald Breedijk (+7860) should be contacted. The measurement schedule will be published in the webcalender on Thursday,  the week before. For first time-users contact Ronald Breedijk (+7860).
Description: The Zeiss LSM510 is a four-channel confocal microscope also capable to perform UV- or MultiPhoton-excitation. Lasers: 405 nm
458 nm, Ar 30 mW
477 nm, Ar 20 mW
488 nm, Ar 30 mW
514 nm, Ar 30 mW
543 nm HeNe laser
561 nm diode laser
633 nm HeNe laser
For more info about laser safety, contact Ronald Breedijk (+7860)
  Objectives: Zeiss Plan-Neofluar, 100 x  /1.30 oil
Zeiss Plan-Apochromat, 63  x /1.40 oil
Zeiss Plan-Neofluar, 40 x  /1.30 oil
Zeiss Plan-Neofluar, 16 x  /0.5 imm
Zeiss C-Apochromat, 63 x  /1.2 W Korr
Zeiss C-Apochromat, 40 x  /1.2 W Korr
Zeiss Plan-Apochromat, 20  x /0.75
Zeiss CP-Apochromat, 10 x  /0.25  Ph1
  Microscope: Axiovert 100M+ mercury and transmission lamp
CFP, FITC and TRITC fluorescence cubes
motorized Marzhauser stage
  Detection filters: Download filter-scheme
  Accessoires: x
  Software: LSM files can be viewed and processed with the LSM AIM software (dongle required), the free LSM  ImageBrowser software or exported to other standard formats. The Loci plugin can be used to import the files into ImageJ or use the Fiji variant.
  Manual: LCAM LSM510 manual (not available yet)
LCAM user rules
Zeiss LSM 510 manual  (hardcopy only)