 |
Location: | LCAM, Room A2.40 |
| Contact person: |  |
|
| 2nd Contact person: |  |
|
| Techniques: | (Semi-)Widefield imaging Confocal imaging Standard confocal techniques like co-localization & FRAP Multi-Photon Excitation (MPE) UV excitation |
|
| Booking: | The LSM510 microscope can be booked by trained users only. To book the microscope Ronald Breedijk (+7860) should be contacted. The measurement schedule will be published in the webcalender on Thursday, the week before. For first time-users contact Ronald Breedijk (+7860). | |
| Description: The Zeiss LSM510 is a four-channel confocal microscope also capable to perform UV- or MultiPhoton-excitation. | Lasers: | 351 nm, Ar UV 80 mW 364 nm, Ar UV 80 mW 458 nm, Ar 30 mW 477 nm, Ar 20 mW 488 nm, Ar 30 mW 514 nm, Ar 30 mW 543 nm, HeNe 0,5 mW 568 nm diode xx mW 633 nm, HeNe 5 mW For more info about laser safety, contact Ronald Breedijk (+7860) |
| Objectives: | Zeiss Plan-Neofluar, 100 x /1.30 oil Zeiss Plan-Apochromat, 63 x /1.40 oil Zeiss Plan-Neofluar, 40 x /1.30 oil Zeiss Plan-Neofluar, 16 x /0.5 imm Zeiss C-Apochromat, 63 x /1.2 W Korr Zeiss C-Apochromat, 40 x /1.2 W Korr Zeiss Plan-Apochromat, 20 x /0.75 Zeiss CP-Apochromat, 10 x /0.25 Ph1 |
|
| Microscope: | Axiovert 100M+ mercury and transmission lamp CFP, FITC and TRITC fluorescence cubes motorized Marzhauser stage |
|
| Detection filters: | Download filter-scheme | |
| Accessoires: | Mira Multiphoton laser Objective + Sample heater |
|
| Software: | LSM files can be viewed and processed with the LSM AIM software (dongle required), the free LSM ImageBrowser software or exported to other standard formats. The Loci plugin can be used to import the files into ImageJ. | |
| Manual: | LCAM LSM510 manual LCAM user rules Zeiss LSM 510 manual (hardcopy only) |