Leica Stellaris 8

Location: Sciencepark room A2.40
Contact person:
2nd Contact person:
Techniques: Confocal imaging of max. 5 channels with standard, FLIM-optimised and/or red color-optimised HyDs (5x)
(Semi-)Widefield imaging
Standard confocal techniques like co-localization & FRAP
FLIM & lifetime gated-imaging
Fast overview imaging
Booking: The microscope can be booked by trained users only. The schedule can be viewed in the webcalender. First time-users should contact Mark Hink.
Lasers: 405 nm diode laser
440-790 pulsed White Light laser (25-80 MHz)
For more info about laser safety, contact Ronald Breedijk (+7860)
  Objectives: HC PL Fluotar 1.25x, NA0.04 (air)
HC Plan Apo 10x, NA0.4 (air)
HC Plan Apo 20x, NA0.75 (air)
HC Plan Apo 63x, NA1.4 (oil)
HC Plan Apo 100x, NA1.4 (oil)
  Filters: Download laser and filter scheme here
  Accessoires: Equiped with Okolab 37oC incubation box with option for CO2
Piezo element for precise z-stacking
Adaptive focusing
  Software: Leica Las-X, images can be imported into ImageJ using the LOCI plugin (automatically recognized in Fiji)
  Manuals: LCAM Leica Stellaris8 Start-up manual
LCAM user rules
LCAM Manual connection to data-server
Las-X helpfiles available via desktop link or via Mark