Category: Research highlight

Research highlight: The Fusarium oxysporum Avr2-Six5 effector pair alters plasmodesmatal exclusion selectivity

Pathogens use effector proteins to manipulate their hosts. During infection of tomato the fungus Fusarium oxysporum secretes the effectors Avr2 and Six5. Whereas Avr2 suffices to trigger I-2-mediated cell death in heterologous systems, both effectors are required for I-2-mediated disease

Research highlight: Shedding light on protein interaction networks

Researchers succeeded for the first time in mapping protein-protein interactions in living developing plant roots. The findings of the international team of the department of Plant Developmental Biology at Wageningen University and Research, the section of Molecular Cytology at the

Research highlight: Spectrally diverse FRET acceptors for mTurquoise2

The performance of Förster Resonance Energy Transfer (FRET) biosensors depends on brightness and photostability, which are dependent on the characteristics of the fluorescent proteins that are employed. Yellow fluorescent protein (YFP) is often used as an acceptor but YFP is

Research highlight: Automated lifetime-based screening and characterization of fluorescent proteins

In biological research, fluorescent proteins (FPs) are widely used in fluorescence microscopy. FPs are genetically encoded fluorophores, which are produced by cells after a noninvasive transfection. These proteins are utilized as markers and bio-sensors to study biological processes in living

Euro-BioImaging is starting Interim Operation

20.05.2016 European life science researchers now have the possibility to use state-of-the-art imaging technologies, which they do not find at their home institutions or among their collaboration partners. All they need to do is to apply to Euro-BioImaging (EuBI) via

LCAM centre among best in Europe

15 December 2015 The van Leeuwenhoek Centre for Advanced Microscopy (LCAM), a collaboration of the Netherlands Cancer Institute, the AMC-UvA and the UvA’s Faculty of Science, is according to Euro-BioImaging one of the leading microscopy centres in Europe. The centre

Research highlight: Subcellular location of signaling proteins determines cell shape

The small GTPase RhoA is best known for its ability to remodel the actin cytoskeleton of cells.  To study the mechanism underlying the spatiotemporal control of RhoA activity by GEFs, single cell imaging was performed with an improved FRET sensor reporting on the nucleotide loading

Research highlight: The effect of sample fixation on fluorescence lifetime

Fluorescence lifetime imaging microscopy (FLIM) can be used to study protein-protein interactions by FRET or to perform lifetime-based multiplexing. Mostly live cell imaging is performed for FLIM imaging, but in combination with (antibody) stainings a fixation step is required. Nevertheless,

Rescan Confocal Microscopy: Winner of Amsterdam Science & Innovation Award 2014

Giulia De Luca wins Amsterdam Science & Innovation Award 2014 11 June 2014 Giulia De Luca’s Re-scan Confocal Microscopy (RCM) method won the 2014 edition of the Amsterdam Science & Innovation Award, an annual award in recognition of innovative ideas

Research highlight: Annexin A4 dynamics

Annexins, found in most eukaryotic species, are cytosolic proteins that are able to bind negatively-charged phospholipids in a calcium-dependent manner. Annexin A4 (AnxA4) has been implicated in diverse cellular processes, including the regulation of exocytosis and ion-transport; however, its precise

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